Tested Applications
| Positive WB detected in | RAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, human lymphoma tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 32 publications below |
| IHC | See 16 publications below |
| IF | See 21 publications below |
| FC | See 1 publications below |
Product Information
66220-1-Ig targets PD-1/CD279 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PD-1/CD279 fusion protein Ag12470 Predict reactive species |
| Full Name | programmed cell death 1 |
| Calculated Molecular Weight | 288 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa, 47-55 kDa |
| GenBank Accession Number | BC074740 |
| Gene Symbol | PD-1 |
| Gene ID (NCBI) | 5133 |
| RRID | AB_2881611 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| IHC protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| IF protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy | ||
Nat Commun m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. | ||
J Exp Clin Cancer Res BAP1 regulates HSF1 activity and cancer immunity in pancreatic cancer | ||
Cancers (Basel) Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma. | ||
Biochem Pharmacol Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT | ||
Int Immunopharmacol EGCG suppresses PD-1 expression of T cells via inhibiting NF-κB phosphorylation and nuclear translocation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Wiesława (Verified Customer) (12-08-2022) | works very well
 |
FH Macarena Lucia (Verified Customer) (10-17-2022) | clear band
|
FH Mona (Verified Customer) (10-17-2021) | Staining of paraffin-embedded lung tissue from hamster. Antigen retrieval was performed with Tris-EDTA (pH 9)
|
