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CD20 Polyclonal antibody

CD20 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 24828-1-AP

Synonyms

Leukocyte surface antigen Leu-16, Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD20 Polyclonal antibody (24828-1-AP)

    WB analysis using 24828-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24828-1-AP (CD20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CD20 Polyclonal antibody (24828-1-AP)

    WB analysis of Raji using 24828-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using CD20 Polyclonal antibody (24828-1-AP)

    IP experiment of Raji using 24828-1-AP

    IP result of anti-CD20 (IP:24828-1-AP, 4ug; Detection:24828-1-AP 1:600) with Raji cells lysate 1440ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human tonsillitis using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human tonsillitis using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human tonsillitis using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human tonsillitis using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human breast cancer using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human breast cancer using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human colon cancer using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human lymphoma using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human lymphoma using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human tonsillitis using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human tonsillitis using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24828-1-AP (MS4A1,CD20 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human colon cancer using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human colon cancer using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD20 Polyclonal antibody (24828-1-AP)

    IHC staining of human colon cancer using 24828-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of human tonsillitis using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of human tonsillitis using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of human tonsillitis using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of human tonsillitis using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of human tonsillitis using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of human tonsillitis using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of human tonsillitis using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of human tonsillitis using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24828-1-AP (CD20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD20 Polyclonal antibody (24828-1-AP)

    IF Staining of Raji using 24828-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (24828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IP detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, Raji cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24828-1-AP targets CD20 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD20 fusion protein Ag17309
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002807
Gene Symbol CD20
Gene ID (NCBI) 931
RRIDAB_2879747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Protocols

Product Specific Protocols
WB protocol for CD20 antibody 24828-1-APDownload protocol
IHC protocol for CD20 antibody 24828-1-APDownload protocol
IF protocol for CD20 antibody 24828-1-APDownload protocol
IP protocol for CD20 antibody 24828-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Immunother Cancer

Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors.

Authors - Wu-Hu Zhang
View Article
humanIHC

Clin Exp Immunol

CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.

Authors - Sirui Qian
View Article
humanWB

Acta Physiol (Oxf)

Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.

Authors - A Zietzer
View Article
humanIHC

Int J Mol Sci

CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing

Authors - Chen Su
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9. Also works by IHC with sodium citrate antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate