CoraLite®594-conjugated PD-1/CD279 Monoclonal antibody

PD-1/CD279 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H4D1

Cat no : CL594-66220

Synonyms

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Validation Data Gallery

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  IF Staining of human lymphoma using CL594-66220

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue using CoraLite®594 PD-1/CD279 antibody (CL594-66220, Clone: 4H4D1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lymphoma using CL594-66220

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue using CoraLite®594 PD-1/CD279 antibody (CL594-66220, Clone: 4H4D1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: human lymphoma tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66220 targets PD-1/CD279 in IF-P applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PD-1/CD279 fusion protein Ag12470
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 288 aa, 32 kDa
• Observed Molecular Weight: 32 kDa, 47-55 kDa
• GenBank Accession Number: BC074740
• Gene Symbol: PD-1
• Gene ID (NCBI): 5133
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).