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PCNA Monoclonal antibody
PCNA Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
10D10E11
Cat no : 60097-1-Ig
Synonyms
"PCNA Antibodies" Comparison
View side-by-side comparison of PCNA antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human gliomas tissue, human tonsillitis tissue, human breast cancer tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, mouse heart tissue |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:250-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 34 publications below |
| IHC | See 18 publications below |
| IF | See 10 publications below |
| IP | See 1 publications below |
Product Information
60097-1-Ig targets PCNA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
| Tested Reactivity | human, mouse, pig, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PCNA fusion protein Ag7416 |
| Full Name | proliferating cell nuclear antigen |
| Calculated molecular weight | 29 kDa/31 kDa |
| Observed molecular weight | 36-38 kDa |
| GenBank accession number | BC000491 |
| Gene symbol | PCNA |
| Gene ID (NCBI) | 5111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCNA antibody 60097-1-Ig | Download protocol |
| IHC protocol for PCNA antibody 60097-1-Ig | Download protocol |
| IF protocol for PCNA antibody 60097-1-Ig | Download protocol |
| IP protocol for PCNA antibody 60097-1-Ig | Download protocol |
| FC protocol for PCNA antibody 60097-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurochem Res Resistin-Inhibited Neural Stem Cell-Derived Astrocyte Differentiation Contributes to Permeability Destruction of the Blood-Brain Barrier. | ||
J Cell Physiol HOXC13-AS promotes breast cancer cell growth through regulating miR-497-5p/PTEN axis. | ||
Int J Mol Med Bradykinin potentially stimulates cell proliferation in rabbit corneal endothelial cells through the ZO‑1/ZONAB pathway. | ||
Biochim Biophys Acta BMP-6 inhibits cell proliferation by targeting microRNA-192 in breast cancer. | ||
Mol Ther Oncolytics A Novel Antitumor Strategy: Simultaneously Inhibiting Angiogenesis and Complement by Targeting VEGFA/PIGF and C3b/C4b. | ||
Biomed Res Int Effect of Quercetin on PC12 Alzheimer's Disease Cell Model Induced by Aβ25-35 and Its Mechanism Based on Sirtuin1/Nrf2/HO-1 Pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Maria (Verified Customer) (08-07-2021) | PCNA in human primary fibroblasts in culture. Passage number 15-25. 15 ug of protein. 4-15% TGX gel. Blocking BSA 3% in PBST. Primary ab 1:1000 in BSA O/N 4ºC. Secondary ab 1:5000 HRP.
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