PCNA Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PCNA Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

10D10E11

Cat no : 60097-1-PBS

Synonyms

Cyclin, PCNA

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Validation Data Gallery

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  WB analysis of HEK-293 using 60097-1-Ig (same clone as 60097-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60097-1-Ig (PCNA Antibody) at various dilution incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60097-1-Ig (same clone as 60097-1-PBS)

  Western blot analysis of PCNA in various cell lines using Proteintech antibody 60097-1-Ig at a dilution of 1:10000 (exposed for 2 seconds) incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60097-1-Ig (same clone as 60097-1-PBS)

  WB result of PCNA antibody (60097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCNA transfected HEK-293 cells. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60097-1-Ig (same clone as 60097-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60097-1-Ig (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 60097-1-Ig (same clone as 60097-1-PBS)

  IP result of anti-PCNA (IP:60097-1-Ig, 4ug; Detection:60097-1-Ig 1:300) with HepG2 cells lysate 3000ug. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60097-1-Ig(PCNA antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60097-1-Ig(PCNA antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 60097-1-Ig (same clone as 60097-1-PBS)

  E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60097-1-Ig (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60097-1-Ig (same clone as 60097-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60097-1-Ig(PCNA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60097-1-PBS targets PCNA in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PCNA fusion protein Ag7416
• Full Name: proliferating cell nuclear antigen
• Calculated Molecular Weight: 29 kDa/31 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC000491
• Gene Symbol: PCNA
• Gene ID (NCBI): 5111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).