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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated PCM1 Polyclonal antibody

PCM1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-19856

Synonyms

PTC4, pericentriolar material 1, PCM-1, PCM 1, hPCM-1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated PCM1 Polyclonal antibody (CL555-19856)

    IF Staining of HeLa using CL555-19856

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 PCM1 antibody (CL555-19856) at dilution of 1:400.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®555-conjugated PCM1 Polyclonal antibody (CL555-19856)

    FC experiment of HepG2 using CL555-19856

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human PCM1 (CL555-19856) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-19856 targets PCM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCM1 fusion protein Ag13929
Full Name pericentriolar material 1
Calculated Molecular Weight 2024 aa, 228 kDa
Observed Molecular Weight 260-280 kDa
GenBank Accession NumberBC000453
Gene Symbol PCM1
Gene ID (NCBI) 5108
RRIDAB_2919636
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

Protocols

Product Specific Protocols
IF protocol for CL555 PCM1 antibody CL555-19856Download protocol
Standard Protocols
Click here to view our Standard Protocols