Validation Data Gallery
|Positive WB detected in||HEK-293 cells, NIH/3T3 cells, HT-1080 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human tonsillitis tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, NIH/3T3 cells, HeLa cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19856-1-AP is PCM1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCM1 fusion protein Ag13929|
|Full Name||pericentriolar material 1|
|Calculated molecular weight||2024 aa, 228 kDa|
|Observed molecular weight||260-280 kDa|
|GenBank accession number||BC000453|
|Gene ID (NCBI)||5108|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).
|Product Specific Protocols|
|WB protocol for PCM1 antibody 19856-1-AP||Download protocol|
|IHC protocol for PCM1 antibody 19856-1-AP||Download protocol|
|IF protocol for PCM1 antibody 19856-1-AP||Download protocol|
|FC protocol for PCM1 antibody 19856-1-AP||Download protocol|
|Click here to view our Standard Protocols|
USP9X regulates centrosome duplication and promotes breast carcinogenesis.
ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
J Cell Biol
A ciliopathy complex builds distal appendages to initiate ciliogenesis.
Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis
Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.
Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Julie (Verified Customer) (01-06-2020)
This antibody works great and reliably with PFA fix & methanol permeabilization.