PAX8 Monoclonal antibody, PBS Only

PAX8 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse , rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H7B3

Cat no : 60145-4-PBS

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

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Validation Data Gallery

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  WB analysis of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60145-4-Ig (same clone as 60145-4-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  WB analysis of human thyroid gland using 60145-4-Ig (same clone as 60145-4-PBS)

  human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IF Staining of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

  Confocal immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using 60145-4-Ig (PAX8 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Phalloidin stains F-actin (Red). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60145-4-PBS targets PAX8 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse , rat samples.

• Tested Reactivity: human, mouse , rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAX8 fusion protein Ag0306
• Full Name: paired box 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC001060
• Gene Symbol: PAX8
• Gene ID (NCBI): 7849
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).