Tested Applications
| Positive WB detected in | SKOV-3 cells, human thyroid gland tissue |
| Positive IHC-Autostainer detected in | human ovary cancer tissue, human renal cell carcinoma tissue, human kidney tissue |
| Positive IHC detected in | human ovary tumor tissue, human renal cell carcinoma tissue, human kidney tissue, human thyroid cancer tissue, mouse kidney tissue, rat kidney tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60145-4-Ig targets PAX8 in WB, IHC, IHC-Autostainer, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0306 Product name: Recombinant human PAX8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-212 aa of BC001060 Sequence: MPHNSIRSGHGGLNQLGGAFVNGRPLPEVVRQRIVDLAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIRPGVIGGSKPKVATPKVVEKIGDYKRQNPTMFAWEIRDRLLAEGVCDNDTVPSVSSINRIIRTKVQQPFNLPMDSCVATKSLSPGHTLIPSSAVTPPESPQSDSLGSTYSINGLLGIAQPGSDKRKMDDSDQDSCRLSIDS Predict reactive species |
| Full Name | paired box 8 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC001060 |
| Gene Symbol | PAX8 |
| Gene ID (NCBI) | 7849 |
| RRID | AB_10643528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q06710 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PAX8 antibody 60145-4-Ig | Download protocol |
| IHC protocol for PAX8 antibody 60145-4-Ig | Download protocol |
| WB protocol for PAX8 antibody 60145-4-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PAX8 Monoclonal antibody (4H7B3), catalog number 60145-4-Ig, has 13 citations published in journals including Nature Cell Biology, Cell Death & Discovery, Developmental Cell, Stem Cell Research & Therapy, Modern Pathology, Frontiers in Cell and Developmental Biology, Scientific Reports, Diagnostic Pathology, Experimental Cell Research, Archives of Pathology & Laboratory Medicine, Human Pathology, and Virchows Archiv. Research fields span renal cell carcinoma pathology, ovarian carcinogenesis, endometrial carcinoma, developmental biology, stem cell biology, and transcriptional regulation.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Segmental specification of the human female fetal reproductive tract revealed by spatiotemporal dynamics. | ||
Cell Death Discov Paired box 8 facilitates the c-MYC related cell cycle progress in TP53-mutation uterine corpus endometrial carcinoma through interaction with DDX5. | ||
Dev Cell Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition | ||
Stem Cell Res Ther Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells | ||
Mod Pathol Xp11.2 translocation renal cell carcinoma with NONO-TFE3 gene fusion: morphology, prognosis, and potential pitfall in detecting TFE3 gene rearrangement. | ||
Front Cell Dev Biol Lgr4 Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway |
Reviews
What customers say
Both reviewers rated this antibody 4 out of 5 stars. One user reported successful immunolabeling in mouse retina tissues using a 1:500 dilution for immunofluorescence. Another confirmed good performance in Western Blot experiments on HepG2 cells at a 1:1000 dilution. Overall, customers find this antibody reliable for both IF and WB applications across different sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kenzo (Verified Customer) (03-28-2024) | It worked well for immunolabeling in mouse retina tissues
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Juan (Verified Customer) (04-18-2019) | It works well in Western Blot experiment.
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