PAX6 Recombinant antibody, PBS Only

PAX6 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241521G1

Cat no : 84345-5-PBS

Synonyms

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

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Validation Data Gallery

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  WB analysis using 84345-5-RR (same clone as 84345-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84345-5-RR (PAX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84345-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84345-5-RR against Human PAX6 were performed. The affinity constant is 40.0 pM.

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Product Information

The immunogen of 84345-5-PBS is PAX6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PAX6 fusion protein Ag2984
• Full Name: paired box 6
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC011953
• Gene Symbol: PAX6
• Gene ID (NCBI): 5080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.