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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated PARP1 Monoclonal antibody

PARP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1D7D4

Cat no : CL555-66520

Synonyms

ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated PARP1 Monoclonal antibody (CL555-66520)

    IF Staining of HeLa using CL555-66520

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 PARP1 antibody (CL555-66520, Clone: 1D7D4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated PARP1 Monoclonal antibody (CL555-66520)

    FC experiment of HeLa using CL555-66520

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human PARP1 (CL555-66520, Clone:1D7D4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66520 targets PARP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PARP1 fusion protein Ag19173
Full Name poly (ADP-ribose) polymerase 1
Calculated Molecular Weight 1014 aa, 113 kDa
Observed Molecular Weight 113-116 kDa, 85-89 kDa
GenBank Accession NumberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
RRIDAB_2919688
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Protocols

Product Specific Protocols
IF protocol for CL555 PARP1 antibody CL555-66520Download protocol
FC protocol for CL555 PARP1 antibody CL555-66520Download protocol
Standard Protocols
Click here to view our Standard Protocols