Biotin-conjugated PARK7/DJ-1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PARK7/DJ-1 Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-11681

Synonyms

DJ 1, DJ1, DJ-1, FLJ27376, FLJ34360, FLJ92274, Oncogene DJ1, PARK7, PARK7,DJ-1, Parkinson disease protein 7, Protein DJ 1

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Validation Data Gallery

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  IHC staining of mouse kidney using Biotin-11681

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using Biotin-11681 (PARK7/DJ-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using Biotin-11681

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using Biotin-11681 (PARK7/DJ-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-11681 targets PARK7/DJ-1 in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARK7/DJ-1 fusion protein Ag2287
• Full Name: Parkinson disease (autosomal recessive, early onset) 7
• Calculated Molecular Weight: 189 aa, 20 kDa
• Observed Molecular Weight: 20 kDa, 25 kDa
• GenBank Accession Number: BC008188
• Gene Symbol: PARK7
• Gene ID (NCBI): 11315
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).