CoraLite® Plus 488-conjugated PAIP1 Monoclonal antibody

PAIP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H9D4

Cat no : CL488-68042

Synonyms

Poly(A)-binding protein-interacting protein 1, PAIP-1, PAIP 1, PABP-interacting protein 1, PABP interacting protein 1

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Validation Data Gallery

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  IF Staining of HeLa using CL488-68042

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PAIP1 antibody (CL488-68042, Clone: 2H9D4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-68042

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PAIP1 (CL488-68042, Clone:2H9D4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68042 targets PAIP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rabbit samples.

• Tested Reactivity: human, mouse, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAIP1 fusion protein Ag16773
• Full Name: poly(A) binding protein interacting protein 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 40-45kDa, 62kDa
• GenBank Accession Number: BC005295
• Gene Symbol: PAIP1
• Gene ID (NCBI): 10605
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C