PAIP1 Monoclonal antibody

PAIP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9D4

Cat no : 68042-1-Ig

Synonyms

PABP interacting protein 1, PAIP 1, PAIP1

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Validation Data Gallery

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  WB analysis using 68042-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68042-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of rabbit brain using 68042-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 68042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, rabbit brain tissue, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68042-1-Ig targets PAIP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rabbit samples.

• Tested Reactivity: Human, mouse, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAIP1 fusion protein Ag16773
• Full Name: poly(A) binding protein interacting protein 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 40-45kDa, 62kDa
• GenBank Accession Number: BC005295
• Gene Symbol: PAIP1
• Gene ID (NCBI): 10605
• RRID: AB_2918784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.