PAIP1 Monoclonal antibody, PBS Only

PAIP1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H9D4

Cat no : 68042-1-PBS

Synonyms

PABP interacting protein 1, PAIP 1, PAIP1

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Validation Data Gallery

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  WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 68042-1-Ig (same clone as 68042-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 68042-1-Ig (same clone as 68042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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Product Information

68042-1-PBS targets PAIP1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rabbit samples.

• Tested Reactivity: Human, mouse, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAIP1 fusion protein Ag16773
• Full Name: poly(A) binding protein interacting protein 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 40-45kDa, 62kDa
• GenBank Accession Number: BC005295
• Gene Symbol: PAIP1
• Gene ID (NCBI): 10605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.