CoraLite® Plus 488-conjugated PADI4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PADI4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17373

Synonyms

PADI5, PAD4, PAD, HL-60 PAD, HL 60 PAD

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Validation Data Gallery

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  IF Staining of A549 using CL488-17373

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 PADI4 antibody (CL488-17373) at dilution of 1:200.

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  FC experiment of HeLa using CL488-17373

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PADI4 (CL488-17373) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17373 targets PADI4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PADI4 fusion protein Ag11068
• Full Name: peptidyl arginine deiminase, type IV
• Calculated Molecular Weight: 663 aa, 74 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC025718
• Gene Symbol: PADI4
• Gene ID (NCBI): 23569
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PADI4, also named as PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues.(PMID: 19183436)