PADI4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PADI4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

39

Cat no : 17373-1-AP

Synonyms

PADI5, PAD4, PAD, HL-60 PAD, HL 60 PAD

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Validation Data Gallery

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  WB analysis of SKOV-3 using 17373-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17373-1-AP (PADI4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 17373-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17373-1-AP (PADI4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 17373-1-AP

  IP result of anti-PADI4 (IP:17373-1-AP, 4ug; Detection:17373-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

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  IHC staining of mouse spleen using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17373-1-AP (PADI4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17373-1-AP (PADI4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 17373-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PADI4 antibody (17373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 17373-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PADI4 antibody (17373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 17373-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PADI4 (17373-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, mouse spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 26 publications below
• IHC: See 7 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

17373-1-AP targets PADI4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PADI4 fusion protein Ag11068
• Full Name: peptidyl arginine deiminase, type IV
• Calculated Molecular Weight: 663 aa, 74 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC025718
• Gene Symbol: PADI4
• Gene ID (NCBI): 23569
• RRID: AB_2878398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PADI4, also named PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases the production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues. (PMID: 19183436)

Publications

Species	Application	Title
rat	WB	Int J Mol Sci; Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis.; Authors - Szilvia Török
mouse	WB	RMD Open; IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.; Authors - Ayano Yahagi
human	WB, IF	Transl Stroke Res; Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage.; Authors - Hanhai Zeng
rat	IF	Int J Mol Sci; Loss of 15-Lipoxygenase in Retinodegenerative RCS Rats; Authors - Andrew James Mead
human	WB	Int Immunopharmacol; IDH1/MDH1 deacetylation promotes NETosis by regulating OPA1 and autophagy; Authors - Yukun Wang
mouse	WB	Virulence; Extracellular trap can be trained as a memory response.; Authors - Yu Gao