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- KD/KO Validated
CoraLite®594-conjugated P53 Polyclonal antibody
P53 Polyclonal Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
human, rat, mouse
IF, FC (Intra)
CoraLite®594 Fluorescent Dye
Cat no : CL594-10442
|Positive IF detected in||A431 cells|
|Positive FC detected in||A431 cells, HepG2 cells|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
CL594-10442 targets P53 in IF, FC (Intra) applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||P53 fusion protein Ag0698|
|Full Name||tumor protein p53|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||53 kDa|
|GenBank accession number||BC003596|
|Gene ID (NCBI)||7157|
|Conjugate||CoraLite®594 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||593 nm / 614 nm|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.|
1. What is p53?
P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.
2. FAQs and p53
a. I fail to detect p53 by western blotting
Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.
b. I fail to detect p53 in some cell lines by western blotting
Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.
c. I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size
p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.