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CoraLite®555-conjugated P53 Recombinant antibody

P53 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2H13

Cat no : CL555-80077

Synonyms

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®555-conjugated P53 Recombinant antibody (CL555-80077)

    IF Staining of HepG2 using CL555-80077

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 P53 antibody (CL555-80077, Clone: 2H13 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®555-conjugated P53 Recombinant antibody (CL555-80077)

    FC experiment of HepG2 using CL555-80077

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human P53 (CL555-80077, Clone:2H13) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-80077 targets P53 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name tumor protein p53
Calculated Molecular Weight 44 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Protocols

Product Specific Protocols
IF protocol for CL555 P53 antibody CL555-80077Download protocol
FC protocol for CL555 P53 antibody CL555-80077Download protocol
Standard Protocols
Click here to view our Standard Protocols