P53 Recombinant monoclonal antibody

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• P53 Recombinant monoclonal antibody (80077-1-RR)

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• KD/KO Validated

This P53 antibody (80077-1-RR) is a rabbit recombinant antibody that targets P53 in WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA, ChIP-qPCR and is suitable for human, rat, zebrafish samples.

Cat No. 80077-1-RR Copied!

Clone No. 2H13

Publications (18)

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Host / Isotype

Rabbit / IgG

Reactivity

human, rat, zebrafish and More (2)

Applications

WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Synonyms

TP53, Antigen NY-CO-13, tumor protein p53, Tumor suppressor p53, Tumour Protein p53

• 

  WB analysis using 80077-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of zebrafish using 80077-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 80077-1-RR

  WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HSC-T6 using 80077-1-RR

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 80077-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SW480 using 80077-1-RR

  SW480 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HT-29 using 80077-1-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of ROS1728 using 80077-1-RR

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovarian cancer using 80077-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue slide using 80077-1-RR (p53 antibody) at dilution of 1:1000 (under 10x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 7.0). Staining was performed on the Epredia 480S Autostainer.​ The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovarian cancer using 80077-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue slide using 80077-1-RR (p53 antibody) at dilution of 1:1000 (under 40x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 7.0). Staining was performed on the Epredia 480S Autostainer. The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 80077-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80077-1-RR (P53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 80077-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 80077-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 80077-1-RR

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  ChIP experiment of HCT 116 using 80077-1-RR

  Chromatin was prepared from HCT 116 cells treated with Etoposide (50 µM, 6 h). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of P53 (80077-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, A431 cells, HSC-T6 cells, HT-29 cells, ROS1728 cells, SW480 cells, zebrafish tissue
• Positive IHC-Autostainer detected in: human colon cancer tissue, human ovarian cancer
• Positive IF/ICC detected in: HepG2 cells, A431 cells
• Positive FC (Intra) detected in: HepG2 cells
• Positive ChIP-qPCR detected in: Etoposide (50 µM, 6h) HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80077-1-RR targets P53 in WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, rat, zebrafish samples.

• Tested Reactivity: human, rat, zebrafish
• Cited Reactivity: human, mouse, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• RRID: AB_2918862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04637
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Publications

What published studies show

P53 Recombinant monoclonal antibody (2H13), catalog number 80077-1-RR, has 18 total citations appearing in J Adv Res, MedComm, Adv Healthc Mater, J Hazard Mater, Antioxidants, World J Gastroenterol, J Ethnopharmacol, Inflammopharmacology, Cell Biol Toxicol, Sci Rep, Toxics, Oncol Rep, Acta Biochim Biophys Sin, Neurotox Res, SLAS Technol, Reprod Toxicol, Cell Biochem Funct, and Front Immunol. Research fields include autophagy, cardiac aging, osteoporosis, cancer, ferroptosis, reproductive biology, and neurotoxicology.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	J Adv Res; Stattic engineering M2 macrophage-derived exosomes mediate autophagy and immune reprogramming for secondary hyperparathyroidism; Authors - Peiting Li
mouse	WB	MedComm (2020); Time-Restricted Feeding Prevents Cardiac Aging by Entraining Gut Microbiota Clock and Promoting Diurnal Rhythm Autophagy in Prediabetic Mice.; Authors - Yuying Zhou
mouse	WB	Adv Healthc Mater; Dual-Functional Nanoparticles to Reverse Osteoblast Senescence and Enhance Calcium Supplementation for Alleviating Senile Osteoporosis; Authors - Caini Yu
human	WB	J Hazard Mater; Perfluorooctanoic acid and perfluorooctanesulfonic acid induce resistance to chemotherapy in colorectal cancer.; Authors - Jingya Liu
mouse	WB	Antioxidants (Basel); Bioactive Peptide C248 of PRDX4 Ameliorates the Function of Testicular Leydig Cells via Mitochondrial Protection.; Authors - Nini Wei
mouse	WB,IF	World J Gastroenterol; Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis; Authors - Xing-Nian Zhou