P53 Recombinant antibody

• Featured Product
• KD/KO Validated

P53 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

2H13

Cat no : 80077-1-RR

Synonyms

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

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Validation Data Gallery

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  WB analysis using 80077-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80077-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80077-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80077-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 80077-1-RR

  WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.

• 

  WB analysis using 80077-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 80077-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 80077-1-RR

  SW480 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 80077-1-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 80077-1-RR

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 80077-1-RR

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of ATDC-5 using 80077-1-RR

  ATDC-5 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 80077-1-RR

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 80077-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80077-1-RR (P53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 80077-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 80077-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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  FC experiment of HepG2 using 80077-1-RR

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: MCF-7 cells, COLO 320 cells, SW480 cells, MDA-MB-231 cells, HCT 116 cells, A431 cells, HT-29 cells, ROS1728 cells, HSC-T6 cells, ATDC-5 cells, Neuro-2a cells, zebrafish tissue, HT-29cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A431 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 2 publications below

Product Information

80077-1-RR targets P53 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• RRID: AB_2918862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Publications

Species	Application	Title
human	WB	Oncol Rep; Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer; Authors - Ke Yang
mouse	WB,IF	Neurotox Res; NRF2 Antagonizes HIV-1 Tat and Methamphetamine-Induced BV2 Cell Ferroptosis by Regulating SLC7A11; Authors - Shucheng Lin
human	WB	Acta Biochim Biophys Sin (Shanghai); Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway; Authors - Danyang Tian
zebrafish	WB	Cell Biol Toxicol; Deficiency of copper responsive gene stmn4 induces retinal developmental defects; Authors - YuanYuan Jing
mouse	WB	Reprod Toxicol; Cadmium disrupt spermatogenic cell cycle via piRNA-DQ717867/p53 pathway; Authors - Jiaoyang Wei
mouse	IF,WB	World J Gastroenterol; Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis; Authors - Xing-Nian Zhou