CoraLite® Plus 488-conjugated P27; KIP1 Monoclonal antibody

P27; KIP1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67355
Clone No.3F12C10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

CDKN1B, P27, 3F12C10, KIP1, Cyclin-dependent kinase inhibitor p27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration:  100 μL, 1000 ug/ml
100 μL, 1000 ug/ml

$479/ 100 μL

In stock in Rosemont, IL. Order within 85 hours and 1 mins to receive by Tuesday, April 8

Freight/Packing: $40.00

Quantity

Buy two full size antibodies and get a third full size primary antibody for free. Use promo code B2G125.


Proteintech Guarantee



Tested Applications

Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67355 targets P27; KIP1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P27; KIP1 fusion protein Ag14634 Predict reactive species
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Calculated Molecular Weight 198 aa, 22 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC001971
Gene Symbol p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
RRIDAB_3672944
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46527
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 P27; KIP1 antibody CL488-67355Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...