CoraLite®650-conjugated Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite®650 Fluorescent Dye

Cat no : CL650-22952

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin

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Validation Data Gallery

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  FC experiment of HepG2 using CL650-22952

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®650 Anti-Human Osteopontin (CL650-22952) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HepG2 cells

For other applications, we recommend the unconjugated version of this antibody, 22952-1-AP

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL650-22952 is Osteopontin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Osteopontin fusion protein Ag19216
• Full Name: secreted phosphoprotein 1
• Calculated molecular weight: 314 aa, 35 kDa
• Observed molecular weight: 70 kDa, 44-66 kDa
• GenBank accession number: BC007016
• Gene symbol: SPP1
• Gene ID (NCBI): 6696
• Conjugate: CoraLite®650 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C