CoraLite®650-conjugated Osteopontin Polyclonal antibody
Osteopontin Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
FC (Intra)
Conjugate
CoraLite®650 Fluorescent Dye
Cat no : CL650-22952
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL650-22952 is Osteopontin Fusion Protein expressed in E. coli.
Tested Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Osteopontin fusion protein Ag19216 |
Full Name | secreted phosphoprotein 1 |
Calculated molecular weight | 314 aa, 35 kDa |
Observed molecular weight | 70 kDa, 44-66 kDa |
GenBank accession number | BC007016 |
Gene symbol | SPP1 |
Gene ID (NCBI) | 6696 |
Conjugate | CoraLite®650 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).
Protocols
Product Specific Protocols | |
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FC protocol for CL650 Osteopontin antibody CL650-22952 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |