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CoraLite® Plus 488-conjugated Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22952

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated Osteopontin Polyclon (CL488-22952)

    FC experiment of HepG2 using CL488-22952

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Osteopontin (CL488-22952) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-22952 is Osteopontin Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteopontin fusion protein Ag19216
Full Name secreted phosphoprotein 1
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 70 kDa, 44-66 kDa
GenBank Accession NumberBC007016
Gene Symbol SPP1
Gene ID (NCBI) 6696
RRIDAB_2919196
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Osteopontin antibody CL488-22952Download protocol
Standard Protocols
Click here to view our Standard Protocols