Mouse Osteopontin Recombinant antibody, PBS Only

Osteopontin Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240250A1

Cat no : 83413-6-PBS

Synonyms

Spp1, Opn, 240250A1

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83413-6-RR (Osteopontin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83413-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83413-6-RR against Mouse Osteopontin were performed. The affinity constant is 2.19 nM.

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Product Information

The immunogen of 83413-6-PBS is Osteopontin Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: secreted phosphoprotein 1
• Calculated Molecular Weight: 32kd
• GenBank Accession Number: NM_001204233.1
• Gene Symbol: Osteopontin
• Gene ID (NCBI): 20750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.