Osteopontin Recombinant antibody

Osteopontin Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240250A1

Cat no : 83413-6-RR

Synonyms

Spp1, Opn, 240250A1

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Validation Data Gallery

• 

  WB analysis of RAW 264.7 using 83413-6-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83413-6-RR (Osteopontin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of RAW 264.7 using 83413-6-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83413-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83413-6-RR against Mouse Osteopontin were performed. The affinity constant is 2.19 nM.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IF/ICC detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83413-6-RR is Osteopontin Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: secreted phosphoprotein 1
• Calculated Molecular Weight: 32kd
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_001204233.1
• Gene Symbol: Osteopontin
• Gene ID (NCBI): 20750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594).