OXCT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

OXCT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1B9

Cat no : 67836-1-PBS

Synonyms

SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1, 3 oxoacid CoA transferase 1

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Validation Data Gallery

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  WB analysis of rat heart using 67836-1-Ig (same clone as 67836-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67836-1-Ig (same clone as 67836-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67836-1-Ig (same clone as 67836-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67836-1-Ig (same clone as 67836-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit brain using 67836-1-Ig (same clone as 67836-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OXCT1 (67836-1-Ig, Clone:1G1B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

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Product Information

67836-1-PBS targets OXCT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

• Tested Reactivity: rabbit, pig, rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OXCT1 fusion protein Ag24792
• Full Name: 3-oxoacid CoA transferase 1
• Calculated Molecular Weight: 520 aa, 56 kDa
• Observed Molecular Weight: 52-56 kDa
• GenBank Accession Number: BC009001
• Gene Symbol: OXCT1
• Gene ID (NCBI): 5019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)