CoraLite® Plus 488-conjugated OLIG2 Polyclonal antibody

OLIG2 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13999

Synonyms

OLIG 2, Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1

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Validation Data Gallery

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  IF Staining of rat brain using CL488-13999

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 OLIG2 antibody (CL488-13999) at dilution of 1:200. DAPI (blue).

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  FC experiment of C6 using CL488-13999

  1X10^6 C6 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human OLIG2 (CL488-13999) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF-P detected in: rat brain tissue
• Positive FC (Intra) detected in: C6 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13999 targets OLIG2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OLIG2 fusion protein Ag5089
• Full Name: oligodendrocyte lineage transcription factor 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC047511
• Gene Symbol: OLIG2
• Gene ID (NCBI): 10215
• RRID: AB_2919084
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

What is the specificity of Olig2?

Oligodendrocyte transcription factor 2 (OLIG2) is expressed by cells found in the central nervous system (CNS) called oligodendrocyte precursor cells (OPCs), which form the myelin sheaths wrapping the axons of neurons in the brain and spinal cord. OPCs differentiate into oligodendrocytes that form the myelin, providing metabolic support and saltatory conduction. OLIG2 is expressed broadly throughout their development to OPCs. This protein can be used to identify many cells of the oligodendrocyte lineage. It is found mostly in the nucleoplasm but also in the cytoplasm.

What is the function of OLIG2?

The basic helix-loop-helix structure of OLIG2 allows it to function as a transcription factor, determining cell fate in the development of neural tissue, where it is located at the pMN domain in the embryonic spinal cord. Expression of OLIG2 causes neural precursors to develop into oligodendrocytes or into motor neurons and expression is then maintained postnatally. OLIG2 co-operates with other factors to cause this differentiation from precursors, although overexpression alone can cause differentiation to the oligodendrocyte lineage.¹ The continued expression of OLIG2 in OPCs indicates an ongoing role in the maintenance of their stemness.

What is the involvement of OLIG2 in disease?

The expression of OLIG2 in glioblastoma, the most common type of malignant brain tumor in adults, is well characterized, where the pathological function is an extension of the normal function. Stem-like cells that propagate the tumor growth have been shown to be OLIG2-expressing, and are one of the key transcription factors involved in the re-programming of differentiated cells of the tumor to stem-like cells.² OLIG2 has also been associated with demyelinating diseases like multiple sclerosis (MS), as OPCs have been shown to be involved in the process of remyelination.³

1.     Liu, Z. et al. Induction of oligodendrocyte differentiation by Olig2 and Sox10: Evidence for reciprocal interactions and dosage-dependent mechanisms. Dev. Biol. 302, 683-693 (2007).

2.     Wegener, A. et al. Gain of Olig2 function in oligodendrocyte progenitors promotes remyelination. Brain 138, 120-35 (2015).

3.     Ettle, B., Schlachetzki, J. C. M. & Winkler, J. Oligodendroglia and Myelin in Neurodegenerative Diseases: More Than Just Bystanders? Mol. Neurobiol. 53, 3046-3062 (2016).