CoraLite® Plus 488-conjugated ODF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ODF2 Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12058

Synonyms

Cenexin, FLJ44866, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber protein 2

---

Validation Data Gallery

• 

  WB analysis using CL488-12058

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-12058 (ODF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12058 targets ODF2 in WB applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ODF2 fusion protein Ag2693
• Full Name: outer dense fiber of sperm tails 2
• Calculated Molecular Weight: 763 aa, 89 kDa
• Observed Molecular Weight: 67-100 kDa
• GenBank Accession Number: BC010629
• Gene Symbol: ODF2
• Gene ID (NCBI): 4957
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.