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CoraLite® Plus 488-conjugated ODF2 Polyclonal antibody

ODF2 Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12058

Synonyms

Cenexin, FLJ44866, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber protein 2



Tested Applications

Positive WB detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12058 targets ODF2 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ODF2 fusion protein Ag2693
Full Name outer dense fiber of sperm tails 2
Calculated Molecular Weight 763 aa, 89 kDa
Observed Molecular Weight 67-100 kDa
GenBank Accession NumberBC010629
Gene Symbol ODF2
Gene ID (NCBI) 4957
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 ODF2 antibody CL488-12058Download protocol
Standard Protocols
Click here to view our Standard Protocols