Validation Data Gallery
|Positive WB detected in||mouse testis tissue, mouse lung tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12058-1-AP targets ODF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ODF2 fusion protein Ag2693|
|Full Name||outer dense fiber of sperm tails 2|
|Calculated molecular weight||763 aa, 89 kDa|
|Observed molecular weight||67-100 kDa|
|GenBank accession number||BC010629|
|Gene ID (NCBI)||4957|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.
|Product Specific Protocols|
|WB protocol for ODF2 antibody 12058-1-AP||Download protocol|
|IHC protocol for ODF2 antibody 12058-1-AP||Download protocol|
|IF protocol for ODF2 antibody 12058-1-AP||Download protocol|
|IP protocol for ODF2 antibody 12058-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.
Mol Biol Cell
Chromosome misalignment is associated with PLK1 activity at cenexin-positive mitotic centrosomes.
Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice‡.
Myosin Id is required for planar cell polarity in ciliated tracheal and ependymal epithelial cells.
Methods Mol Biol
Analyzing Centrioles and Cilia by Expansion Microscopy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jay (Verified Customer) (12-02-2019)
Methanol Fixation.Immunostaining result shows that Odf2 specifically marks the mother centriole in ciliated NIH3T3 cell (Odf2;red, GT335;green for primary cilia, DAPI;blue for nucleus).