ODF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ODF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

41

Reviews (1)

Cat no : 12058-1-AP

Synonyms

ODF2/1, Cenexin

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Validation Data Gallery

• 

  WB analysis of mouse testis using 12058-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12058-1-AP (ODF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12058-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12058-1-AP (ODF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12058-1-AP

  IP result of anti-ODF2 (IP:12058-1-AP, 5ug; Detection:12058-1-AP 1:500) with mouse testis tissue lysate 8000ug.

• 

  IHC staining of human breast cancer using 12058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12058-1-AP (ODF2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12058-1-AP (ODF2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12058-1-AP

  Immunofluorescent analysis of HeLa cells using 12058-1-AP (ODF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse lung tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 25 publications below

Product Information

12058-1-AP targets ODF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ODF2 fusion protein Ag2693
• Full Name: outer dense fiber of sperm tails 2
• Calculated Molecular Weight: 763 aa, 89 kDa
• Observed Molecular Weight: 67-100 kDa
• GenBank Accession Number: BC010629
• Gene Symbol: ODF2
• Gene ID (NCBI): 4957
• RRID: AB_2156630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.

Publications

Species	Application	Title
mouse	IF	Nature; Centrosome anchoring regulates progenitor properties and cortical formation.; Authors - Wei Shao
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
mouse	IF	Cell; Structurally distinct ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility.; Authors - Jean-Ju Chung
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
		Dev Cell; Rab11 endosomes contribute to mitotic spindle organization and orientation.; Authors - Heidi Hehnly
human	WB	J Exp Med; A DNAH17 missense variant causes flagella destabilization and asthenozoospermia.; Authors - Beibei Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jay (Verified Customer) (12-02-2019)

Methanol Fixation.Immunostaining result shows that Odf2 specifically marks the mother centriole in ciliated NIH3T3 cell (Odf2;red, GT335;green for primary cilia, DAPI;blue for nucleus).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse NIH3T3

ODF2 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse NIH3T3 (Cat no:12058-1-AP)

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