CoraLite® Plus 488-conjugated OCIAD1 Monoclonal antibody

• Featured Product
• KD/KO Validated

OCIAD1 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C10C3

Cat no : CL488-66698

Synonyms

Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ

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Validation Data Gallery

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  IF Staining of human liver cancer using CL488-66698

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 OCIAD1 antibody (CL488-66698, Clone: 1C10C3 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL488-66698

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 OCIAD1 antibody (CL488-66698, Clone: 1C10C3 ) at dilution of 1:200.

• 

  IF Staining of HeLa using CL488-66698

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 OCIAD1 antibody (CL488-66698, Clone: 1C10C3 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66698 targets OCIAD1 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: OCIAD1 fusion protein Ag9977
• Full Name: OCIA domain containing 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC003409
• Gene Symbol: OCIAD1
• Gene ID (NCBI): 54940
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).