Ninein Recombinant antibody, PBS Only (Detector)

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  WB analysis of HeLa using 84937-1-RR (same clone as 84937-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84937-1-RR (Ninein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84937-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01704-2

  Sandwich ELISA standard curve of MP01704-2, Human Ninein Recombinant Matched Antibody Pair - PBS only. 84937-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3688. 84937-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL.

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  Sample test of MP01704-2

  The mean Ninein concentration was determined to be 105.0 pg/mL in HeLa cell extract based on a 1.0 mg/mL extract load, 70.8 pg/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 76.3 pg/mL in NCCIT cell extract based on a 1.9 mg/mL extract load and 72.4 pg/mL in HT-29 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP01704-2

  Cytometric bead array standard curve of MP01704-2, Ninein Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84937-3-PBS. Detection antibody: 84937-1-PBS. Standard: Ag3688. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84937-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84937-1-RR against Human Ninein were performed. The affinity constant is 0.172 nM.

Ninein Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84937-1-PBS

Clone No.242525B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein

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Product Information

84937-1-PBS targets Ninein as part of a matched antibody pair:

MP01704-2: 84937-3-PBS capture and 84937-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Ninein fusion protein Ag3688
• Full Name: ninein (GSK3B interacting protein)
• Calculated Molecular Weight: 2090 aa, 243 kDa
• Observed Molecular Weight: 240-255 kDa
• GenBank Accession Number: BC034708
• Gene Symbol: Ninein
• Gene ID (NCBI): 51199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N4C6
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.