HeLa cells were subjected to SDS PAGE followed by western blot with 84937-1-RR (Ninein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 84937-1-RR (Ninein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 84937-1-RR
Biolayer interferometry (BLl) kinetic assays of 84937-1-RR against Human Ninein were performed. The affinity constant is 0.172 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.
HeLa cells were subjected to SDS PAGE followed by western blot with 84937-1-RR (Ninein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 84937-1-RR
Biolayer interferometry (BLl) kinetic assays of 84937-1-RR against Human Ninein were performed. The affinity constant is 0.172 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Ninein Recombinant antibody
Catalog Number
84937-1-RR
Citations
-
Dilutions
WB : 1:1000-1:6000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
