NeutraKine® IL-10 Monoclonal antibody, PBS Only (Capture)

NeutraKine® IL-10 Monoclonal Antibody for IHC, ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4F5

Cat no : 69018-1-PBS

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, NeutraKine® IL-10, TGIF

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50035-1

  Sandwich ELISA standard curve of MP50035-1, IL-10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69018-1-PBS. Detection antibody: 69018-2-PBS. Standard: HZ-1145. Range: 15.6-1000 pg/mL.

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  Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

  Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

• 

  Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)

  Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 69018-1-Ig (same clone as 69018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 69018-1-Ig (same clone as 69018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

• 

  ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.

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Product Information

69018-1-PBS targets NeutraKine® IL-10 as part of a matched antibody pair:

MP50035-1: 69018-1-PBS capture and 69018-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IL-10 protein HZ-1145
• Full Name: interleukin 10
• Gene Symbol: IL-10
• Gene ID (NCBI): 3586
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.