NeutraKine® IL-10 Monoclonal antibody

NeutraKine® IL-10 Monoclonal Antibody for IHC, ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

IHC, IF, Neutralization, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1E4F5

Cat no : 69018-1-Ig

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, NeutraKine® IL-10, TGIF

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Validation Data Gallery

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  Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig

  Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL.

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  Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig

  Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis).

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  IHC staining of human tonsillitis using 69018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 69018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 69018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 69018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  ELISA experiment of Recombinant protein using 69018-1-Ig

  Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Neutralization

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

69018-1-Ig targets NeutraKine® IL-10 in IHC, IF, Neutralization, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IL-10 protein HZ-1145
• Full Name: interleukin 10
• Gene Symbol: IL-10
• Gene ID (NCBI): 3586
• RRID: AB_2918838
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.

Publications

Species	Application	Title
mouse	IHC	Front Cell Dev Biol; Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway.; Authors - Balun Li
mouse	IHC	Int J Biol Sci; NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.; Authors - Rui Bai
human	Neutralization,IF	Gut Microbes; Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics; Authors - Li-Wei Xie
mouse		Cell Metab; Myeloid beta-arrestin 2 depletion attenuates metabolic dysfunction-associated steatohepatitis via the metabolic reprogramming of macrophages; Authors - Xiaoli Wei

Related Products

NeutraControl IL-10 Monoclonal antibody

69518-1-Ig