CoraLite® Plus 488-conjugated NUP50 Monoclonal antibody

NUP50 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D5A7

Cat no : CL488-67001

Synonyms

PRO1146, Nucleoporin Nup50, nucleoporin 50kDa, Nuclear pore-associated protein 60 kDa-like, 50 kDa nucleoporin

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Validation Data Gallery

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  FC experiment of HL-60 using CL488-67001

  1X10^6 HL-60 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NUP50 (CL488-67001, Clone:1D5A7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: HL-60 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67001 targets NUP50 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NUP50 fusion protein Ag15144
• Full Name: nucleoporin 50kDa
• Calculated Molecular Weight: 468 aa, 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC028125
• Gene Symbol: NUP50
• Gene ID (NCBI): 10762
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.