CoraLite® Plus 488-conjugated NRBP2 Monoclonal antibody

NRBP2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4F10H7

Cat no : CL488-66687

Synonyms

DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-66687

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488-conjugated NRBP2 antibody (CL488-66687, Clone: 4F10H7 ) at dilution of 1:100.

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  IF Staining of HeLa using CL488-66687

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66687 (NRBP2 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66687 targets NRBP2 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NRBP2 fusion protein Ag17011
• Full Name: nuclear receptor binding protein 2
• Calculated Molecular Weight: 501 aa, 58 kDa
• Observed Molecular Weight: 58 kDa, 33 kDa
• GenBank Accession Number: BC113873
• Gene Symbol: NRBP2
• Gene ID (NCBI): 340371
• RRID: AB_2883367
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.