Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 66687-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 66687-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 66687-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66687-1-Ig (NRBP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66687-1-Ig (NRBP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, pig brain tissue, A549 cells, HeLa cells, rat brain tissue, mouse brain tissue
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunohistochemistry (IHC)
IHC : 1:150-1:600
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
66687-1-Ig targets NRBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.
Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 66687-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66687-1-Ig (NRBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 66687-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 66687-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66687-1-Ig (NRBP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HEK-293 using 66687-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66687-1-Ig (NRBP2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
