NOTCH2 Recombinant antibody

NOTCH2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241162A2

Cat no : 84074-4-RR

Synonyms

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Validation Data Gallery

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  WB analysis using 84074-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-87 MG cells, HEK-293 cells, A431 cells, HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84074-4-RR is NOTCH2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NOTCH2 fusion protein Ag29413
• Full Name: Notch homolog 2 (Drosophila)
• Calculated Molecular Weight: 265 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_024408
• Gene Symbol: NOTCH2
• Gene ID (NCBI): 4853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).