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NOTCH2 Recombinant antibody, PBS Only

NOTCH2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241162A2

Cat no : 84074-4-PBS

Synonyms

Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOTCH2 Recombinant antibody (84074-4-RR)

    WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NOTCH2 Recombinant antibody (84074-4-RR)

    WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

Product Information

84074-4-PBS targets NOTCH2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NOTCH2 fusion protein Ag29413
Full Name Notch homolog 2 (Drosophila)
Calculated Molecular Weight 265 kDa
Observed Molecular Weight 110 kDa, 265kDa
GenBank Accession NumberNM_024408
Gene Symbol NOTCH2
Gene ID (NCBI) 4853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).