NOTCH2 Recombinant antibody, PBS Only

• 

  WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84074-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84074-4-RR against Human NOTCH2 were performed. The affinity constant is 1.86 nM.

NOTCH2 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 84074-4-PBS

Clone No.241162A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2

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Product Information

84074-4-PBS targets NOTCH2 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NOTCH2 fusion protein Ag29413
• Full Name: Notch homolog 2 (Drosophila)
• Calculated Molecular Weight: 265 kDa
• Observed Molecular Weight: 110 kDa, 265kDa
• GenBank Accession Number: NM_024408
• Gene Symbol: NOTCH2
• Gene ID (NCBI): 4853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q04721
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).