CoraLite® Plus 488-conjugated NOC2L Polyclonal antibody

NOC2L Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28509

Synonyms

Protein NOC2 homolog, Nucleolar complex protein 2 homolog, Novel INHAT repressor, NOC2-like protein, NIR

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Validation Data Gallery

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  IF Staining of U2OS using CL488-28509

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 NOC2L antibody (CL488-28509) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of U2OS using CL488-28509

  1x10^6 U2OS cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated NOC2L Polyclonal antibody (CL488-28509)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28509 targets NOC2L in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOC2L fusion protein Ag26952
• Full Name: nucleolar complex associated 2 homolog (S. cerevisiae)
• Calculated Molecular Weight: 749 aa, 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC003555
• Gene Symbol: NOC2L
• Gene ID (NCBI): 26155
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).