CoraLite®594-conjugated NLRX1 Polyclonal antibody

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  IF Staining of HepG2 using CL594-17215

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 NLRX1 antibody (CL594-17215) at dilution of 1:800.

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  IF Staining of A549 using CL594-17215

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 NLRX1 antibody (CL594-17215) at dilution of 1:800.

• Featured Product
• KD/KO Validated

NLRX1 Polyclonal Antibody for IF/ICC

Cat No. CL594-17215

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Nucleotide-binding oligomerization domain protein 9, Nucleotide-binding oligomerization domain protein 5, NOD9, NOD5, NLRX 1

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-17215 targets NLRX1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NLRX1 fusion protein Ag11000
• Full Name: NLR family member X1
• Calculated Molecular Weight: 975 aa, 108 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC013199
• Gene Symbol: NLRX1
• Gene ID (NCBI): 79671
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86UT6
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NLRX1 (Nucleotide-binding oligomerization domain, leucine-rich repeat containing X1) is a member of the NOD-like receptor (NLR) family and is unique among NLRs due to its localization to the mitochondrial matrix. NLRX1 is a negative regulator of innate immunity, particularly in viral infections. It interacts with the mitochondrial antiviral signaling protein (MAVS) to attenuate antiviral responses. NLRX1 has been implicated in various diseases, including multiple sclerosis, colorectal cancer, and ischemia-reperfusion injury. Its role in controlling inflammation and mitochondrial function makes it a potential therapeutic target.