NLRC5 Recombinant antibody, PBS Only (Capture)

NLRC5 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240338D8

Cat no : 83384-2-PBS

Synonyms

NOD4, NOD27, CLR16.1, Caterpiller protein 16.1, 240338D8

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Validation Data Gallery

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  IF Staining of THP-1 using 83384-2-RR (same clone as 83384-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NLRC5 antibody (83384-2-RR, Clone: 240338D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83384-2-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 83384-2-RR (same clone as 83384-2-PBS)

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug NLRC5 Recombinant antibody (83384-2-RR, Clone:240338D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83384-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00402-3

  Cytometric bead array standard curve of MP00402-3, NLRC5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83384-2-PBS. Detection antibody: 83384-3-PBS. Standard: Ag34508. Range: 1.56-100 ng/mL.

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  Affinity and Kinetic Characterization of 83384-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83384-2-RR against Human NLRC5 were performed. The affinity constant is below 1 pM.

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Product Information

83384-2-PBS targets NLRC5 as part of a matched antibody pair:

MP00402-3: 83384-2-PBS capture and 83384-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NLRC5 fusion protein Ag34508
• Full Name: NLR family, CARD domain containing 5
• Calculated Molecular Weight: 204 kDa
• GenBank Accession Number: BC063566
• Gene Symbol: NLRC5
• Gene ID (NCBI): 84166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The nucleotide-binding domain leucine-rich repeat containing (NLR) family of proteins is mainly involved in microbial pathogen recognition, inflammatory responses, and cell death. NLRC5, the largest member of the NLR family, is currently receiving an increasing level of attention. NLRC5 plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways.