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  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using NLRC5 Recombinant antibody (83384-2-RR)

    IF Staining of THP-1 using 83384-2-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NLRC5 antibody (83384-2-RR, Clone: 240338D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of THP-1 cells using NLRC5 Recombinant antibody (83384-2-RR)

    FC experiment of THP-1 using 83384-2-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug NLRC5 Recombinant antibody (83384-2-RR, Clone:240338D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83384-2-RR

    Affinity and Kinetic Characterization of 83384-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83384-2-RR against Human NLRC5 were performed. The affinity constant is below 1 pM.

NLRC5 Recombinant antibody

NLRC5 {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83384-2-RR
Clone No.240338D8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

NOD4, NOD27, CLR16.1, Caterpiller protein 16.1, 240338D8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83384-2-RR targets NLRC5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NLRC5 fusion protein Ag34508 Predict reactive species
Full Name NLR family, CARD domain containing 5
Calculated Molecular Weight204 kDa
GenBank Accession NumberBC063566
Gene Symbol NLRC5
Gene ID (NCBI) 84166
RRIDAB_3671040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The nucleotide-binding domain leucine-rich repeat containing (NLR) family of proteins is mainly involved in microbial pathogen recognition, inflammatory responses, and cell death. NLRC5, the largest member of the NLR family, is currently receiving an increasing level of attention. NLRC5 plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways.

Protocols

Product Specific Protocols
IF protocol for NLRC5 antibody 83384-2-RRDownload protocol
FC protocol for NLRC5 antibody 83384-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols