Anti-Rat NKp46/NCR1 Rabbit Recombinant Antibody

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  FC experiment of rat splenocytes using 98250-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat NKp46/NCR1 Rabbit Recombinant Antibody (98250-1-RR, Clone: 241811E9) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of rat splenocytes using 98250-1-RR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat NKp46/NCR1 Rabbit Recombinant Antibody (98250-1-RR, Clone: 241811E9) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98250-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98250-1-RR against Rat NKp46/NCR1 were performed. The affinity constant is 0.425 nM.

NKp46/NCR1 {ptg:Brand} Recombinant Antibody for FC

Cat No. 98250-1-RR

Clone No.241811E9

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Synonyms

Ly94, Kilr1, CD335, Ar1, Activating receptor 1

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98250-1-RR targets NKp46/NCR1 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 37kDa
• GenBank Accession Number: NM_057199.1
• Gene Symbol: NKp46/NCR1
• Gene ID (NCBI): 117547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Z0H5
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).