NHE1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NHE1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D9F3

Cat no : 67363-1-PBS

Synonyms

APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1

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Validation Data Gallery

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  WB analysis using 67363-1-Ig (same clone as 67363-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67363-1-Ig (NHE1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67363-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67363-1-Ig (same clone as 67363-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67363-1-Ig (NHE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67363-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67363-1-Ig (same clone as 67363-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67363-1-Ig (NHE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67363-1-PBS in a different storage buffer formulation.

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Product Information

67363-1-PBS targets NHE1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NHE1 fusion protein Ag14329
• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1
• Calculated Molecular Weight: 815 aa, 91 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC012121
• Gene Symbol: NHE1
• Gene ID (NCBI): 6548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NHE1, also named as SLC9A1, is involved in pH regulation that can eliminate acids generated by active metabolism and counter adverse environmental conditions. NHE1 plays an important role in signal transduction. It has been shown that NHE1 participates in the development and progression of human breast cancer, gastric cancer and others. NHE1 has some isoforms with 91 kDa , 110 kDa (phosphoglycoprotein) and 210 kDa (dimeric protein). (PMID:28098891, 27751915, 8300588)