NHE1 Monoclonal antibody

• Featured Product
• KD/KO Validated

NHE1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

3D9F3

Cat no : 67363-1-Ig

Synonyms

APNH, APNH1, FLJ42224, Na(+)/H(+) exchanger 1, NHE 1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1

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Validation Data Gallery

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  WB analysis using 67363-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67363-1-Ig (NHE1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  IHC staining of human liver using 67363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67363-1-Ig (NHE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67363-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67363-1-Ig (NHE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, NCI-H1299 cells, HEK-293 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, PC-12 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

67363-1-Ig targets NHE1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NHE1 fusion protein Ag14329
• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1
• Calculated Molecular Weight: 815 aa, 91 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC012121
• Gene Symbol: NHE1
• Gene ID (NCBI): 6548
• RRID: AB_2882615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHE1, also named as SLC9A1, is involved in pH regulation that can eliminate acids generated by active metabolism and counter adverse environmental conditions. NHE1 plays an important role in signal transduction. It has been shown that NHE1 participates in the development and progression of human breast cancer, gastric cancer and others. NHE1 has some isoforms with 91 kDa , 110 kDa (phosphoglycoprotein) and 210 kDa (dimeric protein). (PMID:28098891, 27751915, 8300588)

Publications

Species	Application	Title
rat	WB	Acta Pharmacol Sin; Direct cardio-protection of Dapagliflozin against obesity-related cardiomyopathy via NHE1/MAPK signaling.; Authors - Ke Lin ; KD Validated
human	IHC	Reproduction; An abnormal LPA/LPAR1-NHE1 axis leads to the autophagy deficiency of trophoblast cells in recurrent spontaneous abortion; Authors - Min Chen
rat	WB,IHC	Acta Biochim Biophys Sin (Shanghai); Puerarin inhibits NHE1 activity by interfering with the p38 pathway and attenuates mitochondrial damage induced by myocardial calcium overload in heart failure rats; Authors - Guopin Pan