NFIL3 Monoclonal antibody

NFIL3 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F4B4

Cat no : 66368-1-Ig

Synonyms

Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4, E4 promoter-binding protein 4

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 66368-1-Ig

  Western blot analysis of NFIL3 in various cell lines and tissues using Proteintech antibody 66368-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of NK92 using 66368-1-Ig

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (66368-1-Ig, Clone:2F4B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue
• Positive FC (Intra) detected in: NK92, NK92 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66368-1-Ig targets NFIL3 in WB, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NFIL3 fusion protein Ag2380
• Full Name: nuclear factor, interleukin 3 regulated
• Calculated Molecular Weight: 462 aa, 51 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC008197
• Gene Symbol: NFIL3
• Gene ID (NCBI): 4783
• RRID: AB_2881748
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).