NFATC1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NFATC1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1B10

Cat no : 66963-1-PBS

Synonyms

Nuclear factor of activated T cells, cytoplasmic 1, NF-ATc1, NF-ATc, NFATc, NFAT2

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Validation Data Gallery

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  WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66963-1-Ig (same clone as 66963-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66963-1-Ig (same clone as 66963-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

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Product Information

66963-1-PBS targets NFATC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NFATC1 fusion protein Ag13014
• Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
• Calculated Molecular Weight: 716aa,78 kDa; 943aa,101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC104753
• Gene Symbol: NFATC1
• Gene ID (NCBI): 4772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).