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NFATC1 Monoclonal antibody
NFATC1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (3)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1E1B10
Cat no : 66963-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, U2OS cells, Daudi cells, Romas cells, Raji cells, A549 cells, MOLT-4 cells, Ramos cells, Karpas-422 cells |
| Positive IHC detected in | human urothelial carcinoma tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 45 publications below |
| IHC | See 1 publications below |
| IF | See 10 publications below |
| IP | See 1 publications below |
Product Information
66963-1-Ig targets NFATC1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NFATC1 fusion protein Ag13014 |
| Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 |
| Calculated Molecular Weight | 716aa,78 kDa; 943aa,101 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC104753 |
| Gene Symbol | NFATC1 |
| Gene ID (NCBI) | 4772 |
| RRID | AB_2882286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NFATC1 antibody 66963-1-Ig | Download protocol |
| IHC protocol for NFATC1 antibody 66963-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer | ||
Mol Ther C5a-C5aR1 axis controls mitochondrial fission to promote podocyte injury in lupus nephritis | ||
Pharmacol Res Targeting CD38/ ADP-ribosyl cyclase as a novel therapeutic strategy for identification of three potent agonists for leukopenia treatment | ||
Cell Mol Biol Lett The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis | ||
Oncogene Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells. | ||
Cancer Lett TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway. |
