CoraLite®488-conjugated NEUROD1 Monoclonal antibody

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  FC experiment of SH-SY5Y using CL488-66691

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®488-conjugated NEUROD1 Monoclonal antibody (CL488-66691, Clone:3E10F1)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NEUROD1 Monoclonal Antibody for FC (Intra)

Cat No. CL488-66691

Clone No.3E10F1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

NEUROD, Class A basic helix-loop-helix protein 3, bHLHa3, 3E10F1

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Tested Applications

• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66691 targets NEUROD1 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NEUROD1 fusion protein Ag27606
• Full Name: neurogenic differentiation 1
• Calculated Molecular Weight: 356 aa, 40 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC009046
• Gene Symbol: NEUROD1
• Gene ID (NCBI): 4760
• RRID: AB_2923755
• Conjugate: CoraLite®488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 491 nm / 516 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13562
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.